Data from: Mapping and analysis of the connectome of sympathetic premotor neurons in the rostral ventrolateral medulla of the rat using a volumetric brain atlas

Spinally projecting neurons in the rostral ventrolateral medulla (RVLM) play a critical role in the generation of vasomotor sympathetic tone and are thought to receive convergent input from neurons at every level of the neuraxis; the factors that determine their ongoing activity remain unresolved. In this study we use a genetically restricted viral tracing strategy to definitively map their spatially diffuse connectome. We infected bulbospinal RVLM neurons with recombinant rabies variant that drives reporter expression in monosynaptically connected input neurons and mapped their distribution using a MRI-based volumetric atlas and a novel image alignment and visualization tool that efficiently translates the positions of neurons captured in conventional photomicrographs to Cartesian coordinates. We identified prominent inputs from well-established neurohumoral and viscero-sympathetic sensory actuators, medullary autonomic and respiratory subnuclei, and supramedullary autonomic nuclei. The majority of inputs lay within the brainstem (88 – 94%), and included putative respiratory neurons in the pre-Bötzinger Complex and post-inspiratory complex that are therefore likely to underlie respiratory-sympathetic coupling. We also discovered a substantial and previously unrecognized input from the region immediately ventral to nucleus prepositus hypoglossi. In contrast, RVLM sympathetic premotor neurons were only sparsely innervated by suprapontine structures including the paraventricular nucleus, lateral hypothalamus, periaqueductal grey and superior colliculus, and we found almost no evidence of direct inputs from the cortex or amygdala. Our approach can be used to quantify, standardize and share complete neuroanatomical datasets, and therefore provides researchers with a platform for presentation, analysis and independent analysis of connectomic data.

Usage Notes

Waxholm Rat Calculation TemplateThis template will help you to translate pixel co-ordinates of cells identified from microscopy images to Waxholm co-ordinates. In needs to be used in conjunction with the AlignNii image anchoring tool, which aligns 2d histological images in the 3d MRI Waxholm rat brain. Access to this tool can be requested from Jan Bjaalie (j.g.bjaalie@medisin.uio.no).Waxholm volumetric atlasThis is the zipped Imaris file that contains the Waxholm MRI dataset, the corresponding segmented dataset, the volumetric segmentation model and all of connectomic data from this paper.Dempsey 2016 RVLM Connectome Resource.zip