Data from: The evolution of polymorphism in the warning coloration of the Amazonian poison frog Adelphobates galactonotus

While intraspecific variation in aposematic signals can be selected for by different predatory responses, their evolution is also contingent on other processes shaping genetic variation. We evaluate the relative contributions of selection, geographic isolation and random genetic drift to the evolution of aposematic color polymorphism in the poison frog Adelphobates galactonotus, distributed throughout eastern Brazilian Amazonia. Dorsal coloration was measured for 111 individuals and genetic data were obtained from 220 individuals at two mitochondrial genes (mtDNA) and 7963 Single Nucleotide Polymorphisms (SNPs). Four color categories were described (brown, blue, yellow, orange) and our models of frog and bird visual systems indicated that each color was distinguishable for these taxa. Using outlier and correlative analyses we found no compelling genetic evidence for color being under divergent selection. A time-calibrated mtDNA tree suggests that the present distribution of dorsal coloration resulted from processes occurring during the Pleistocene. Separate phylogenies based on SNPs and mtDNA resolved the same well supported clades, each containing different colored populations. Ancestral character state analysis provided some evidence for evolutionary transitions in color type. Genetic structure was more strongly associated with geographic features, than color category, suggesting that the distribution of color is explained by localized processes. Evidence for geographic isolation together with estimates of low effective population size implicates drift as playing a key role in color diversification. Our results highlight the relevance of  considering the neutral processes involved with the evolution of traits with important fitness consequences.

Methods

Genomic DNA extracted from 186 tissue samples was sent to Diversity Array Tecnologies where SNP discovery and genotyping was performed using the standart DartSeqTM protocol. The provided dataset was further using the following criteria: We only included SNPs with a call rate of >98 % (i.e. less than 2 % missing data), where the average read depth for both alleles was >5 and the Minor Allele Frequency (MAF) > 0.05. We retained only a single SNP per fragment to avoid creating a dataset containing closely linked loci. For analyses were populations with five or more samples.

Usage Notes

Dataset containing: 1) Geographic coordinates of sampling locations. 2) Adelphobates galactonotus filtered SNPs.