Macquarie University
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Cell-type specific identification by different glycan features in neuroinflammation

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posted on 2022-10-18, 03:41 authored by Sagar DalalSagar Dalal

The central nervous system (CNS) has specialised and diverse cell populations. Identifying cell-specific molecular signatures may reveal more comprehensive information about neurological pathways and CNS complexity. Glycosylation is the most common functional post-translation modification (PTM) in the brain and identifying cell-type dependent glycan features helps provide a better understanding of their role in brain cell intercellular interactions. In this study, we identified plasma membrane glycan features of three mammalian brain cell lines; BV2 (microglia), U87-MG (astrocytes) and differentiated SH-SY5Y (neurons) under lipopolysaccharide (LPS) stimulated inflammation. A panel of 9 lectins used here for histochemistry and fluorescence microscopy helps visually identify distinct cell-specific glycan features followed by quantification by FLD-HPLC and PGC-LC-MS/MS for released glycosaminoglycan disaccharides and N-glycans. Our results revealed cell type specific glycan expression, with cell selective binding of Sambucus nigra agglutinin (SNA) and Wisteria floribunda agglutinin (WFA) lectins. SNA lectin binding and LC-MS/MS revealed the absence of α-2,6 linked sialic acid glycans on U87-MG cells. BV2 microglia showed a significant decrease in the glycosaminoglycan, heparan sulphate over time under LPS inflammatory conditions. In summary, this study advances our knowledge of the complexity of brain cell glycan feature expression in a cell type specific and inflammation driven context.


Table of Contents

Chapter 1: Introduction -- Chapter 2: Materials and methods -- Chapter 3: Result -- Chapter 4: Discussion -- Chapter 5: Summary and conclusion -- Supplementary


A thesis submitted to Macquarie University for the degree of Masters of Research (MRes)

Awarding Institution

Macquarie University

Degree Type

Thesis MRes


Thesis (MRes), Macquarie University, Faculty of Science and Engineering, 2021

Department, Centre or School

Department of Molecular Sciences

Year of Award


Principal Supervisor

Lindsay Parker

Additional Supervisor 1

Edward Moh


Copyright: Sagar Dalal Copyright disclaimer:




71 pages