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Class 1 integrons and their impact on the mobility of antibiotic resistance in clinical environments

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thesis
posted on 28.03.2022, 18:35 by Miranda Christopher
Antibiotic resistance in pathogenic bacteria is becoming an increasingly important issue in the management of infectious diseases. Mortalities resulting from the inability to treat infections caused by resistant organisms have been recorded in significant numbers around the world. Mobile genetic elements such as integrons, transposons and gene cassettes, along with the lateral gene transfer which occurs between them, are largely responsible for the spread of resistance genes amongst pathogenic Gram negative bacteria. Integrons are gene expression systems capable of capturing and disseminating mobile gene elements such as gene cassettes. These gene cassettes, when associated with clinical integrons, predominantly code for antibiotic resistance genes. The fact that integrons allow both the integration and excision of multiple gene cassettes leads to the efficient spread of multi-drug resistance amongst pathogenic bacteria. This project investigates antibiotic resistance in Escherichia coli by examining class 1 integrons within several contexts. A collection of 718 E. coli isolates were obtained from clinical sources, primarily originating from urinary tract infections. These isolates were typed for antibiotic resistance capabilities and tested for class 1 and 2 integron presence. Integron cassette arrays were characterized, sequenced and mapped. In the collection, 23% of isolates carried class 1 integrons, while 3% carried a class 2 integron. Eight distinct cassette arrays were detected, consisting of gene cassettes solely from the aadA and dfrA families. Since identical integrons and cassette arrays appeared in a range of E. coli phylotypes and clonal lines, there appears to be significant and ongoing lateral gene transfer between E. coli lineages.

History

Notes

A thesis submitted in fulfillment of the requirements of the degree of Master of Philosophy" "December 2013 Bibliography:pages 98-115

Awarding Institution

Macquarie University

Degree Type

Thesis masters research

Degree

MPhil, Macquarie University, Faculty of Science, Department of Biological Sciences

Department, Centre or School

Department of Biological Sciences

Year of Award

2014

Principal Supervisor

Michael Gillings

Additional Supervisor 1

Hatch Strokes

Rights

Copyright Miranda Christopher 2014. Copyright disclaimer: http://www.copyright.mq.edu.au

Language

English

Extent

1 online resource (152 pages) illustrations (some coloured), graphs, charts

Former Identifiers

mq:40880 http://hdl.handle.net/1959.14/986311 2442348