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Developing drug screening methods on a zebrafish model of motor neurone disease

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posted on 2022-03-28, 21:42 authored by Madelaine Tym
The FUS protein is a nuclear DNA/RNA binding protein that regulates gene expression and that when mutations occur in this protein it can aggregate and contribute to MND pathology. This project aimed to test the effect of treating transgenic zebrafish larvae expressing mutant FUS-R521C-GFP protein with drugs that induce the autophagy pathway. Different methodologies were used to examine whether these drugs can aid removal of FUS aggregates and decrease levels of mutant FUS protein. Treatments with spermidine saw a decrease in FUS-R521C-GFP protein with western blot analysis and a decrease in aggregation with flow cytometry analysis.


Table of Contents

1 Introduction -- 2 Methods -- 3 Results -- Discussion.


Theoretical thesis. Bibliography pages 44-50

Awarding Institution

Macquarie University

Degree Type

Thesis MRes


MRes, Macquarie University, Faculty of Medicine and Health Sciences, Department of Biomedical Sciences

Department, Centre or School

Department of Biomedical Sciences

Year of Award


Principal Supervisor

Angela Laird


Copyright 2019




1 online resource (vii, 51 pages) illustrations

Former Identifiers

mq:72306 http://hdl.handle.net/1959.14/1283482