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Developing drug screening methods on a zebrafish model of motor neurone disease

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thesis
posted on 28.03.2022, 21:42 authored by Madelaine Tym
The FUS protein is a nuclear DNA/RNA binding protein that regulates gene expression and that when mutations occur in this protein it can aggregate and contribute to MND pathology. This project aimed to test the effect of treating transgenic zebrafish larvae expressing mutant FUS-R521C-GFP protein with drugs that induce the autophagy pathway. Different methodologies were used to examine whether these drugs can aid removal of FUS aggregates and decrease levels of mutant FUS protein. Treatments with spermidine saw a decrease in FUS-R521C-GFP protein with western blot analysis and a decrease in aggregation with flow cytometry analysis.

History

Table of Contents

1 Introduction -- 2 Methods -- 3 Results -- Discussion.

Notes

Theoretical thesis. Bibliography pages 44-50

Awarding Institution

Macquarie University

Degree Type

Thesis MRes

Degree

MRes, Macquarie University, Faculty of Medicine and Health Sciences, Department of Biomedical Sciences

Department, Centre or School

Department of Biomedical Sciences

Year of Award

2019

Principal Supervisor

Angela Laird

Rights

Copyright 2019

Language

English

Extent

1 online resource (vii, 51 pages) illustrations

Former Identifiers

mq:72306 http://hdl.handle.net/1959.14/1283482