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Effect of Seaweed Extract on the Binding of Oral Microbes to Saliva

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thesis
posted on 2023-08-22, 06:00 authored by Vishnu Nallaperumal

Microorganisms bind to the glycans attached to proteins, thus the goal of this study was to investigate the role of glycans in the adherence of specific microbial strains linked to the oral microbiome or oral infections. This was investigated by microbial adhesion assay on polyvinylidene fluoride (PVDF) membrane plate with saliva proteins containing N-linked and O-linked glycans immobilized in the wells. Common microorganisms in the oral cavity were selected. The results will help in the understanding of microorganisms binding profiles to the salivary glycoproteins. The effect of saliva from people having different ABO-blood groups on microbial binding was investigated. The potential use of polysaccharides extracted from seaweeds to bind to the microbes to reproduce the role of salivary glycoproteins in populating the oral microbiome was also studied. The seaweed extracts were produced by the industrial partner Venus Shell Systems®, through a series of extraction procedures. The microbes were binding differently to ABO saliva types and responded differently after preincubation with seaweed extracts. The future scope of this study will be to find the best seaweed extract that can mimic the role of saliva in the binding of the beneficial microbiome whilst removing pathogens from populating the dry mouth of cancer patients undergoing chemotherapy.

History

Table of Contents

1. Introduction -- 2. Materials and Methods -- 3. Results -- 4. Discussions -- 5. Conclusion and Future Scope -- 6. References -- 7. Appendix

Awarding Institution

Macquarie University

Degree Type

Thesis MRes

Department, Centre or School

School of Natural Sciences

Year of Award

2023

Principal Supervisor

Nicolle Packer

Additional Supervisor 1

Liisa Kautto

Rights

Copyright: The Author Copyright disclaimer: https://www.mq.edu.au/copyright-disclaimer

Language

English

Extent

83 pages

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