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Effects of common agrochemicals on the health and function of honey bee colonies
thesisposted on 2022-03-28, 17:16 authored by Théotime Colin
Pesticides have come under scrutiny in the last two decades for their effects on European honey bees. The effects of the highest doses of insecticides encountered on crops by honey bees are often lethal and well acknowledged. There is, however, much debate surrounding the potential consequences of exposure to smaller amounts of these pesticides for bee colonies. Field studies at these levels are scarce and often report contradictory results across environments. Due to technical constraints, these studies generally lack the precision and temporal resolution necessary to elucidate what causes the effects of a pesticide to vary across environments. Much attention has been given to the neonicotinoid insecticides, because of early reports of colony failure associated with elevated amounts of these pesticides in dead worker bees. The pesticides used by beekeepers within hives against the parasitic mite Varroa destructor have, however, received much less attention. Controlled experiments on the effect of these treatments on whole bee colonies are scarce, even though they are almost ubiquitous in hives. To lift some of the technical constraints specific to honeybee studies, we developed a method to automatically and continuously measure the daily variations of the weight of hives, such that we can extract from these data the amount of nectar collected daily and indices of foraging intensity. We then developed a new analytical method to quickly measure the area of capped brood and capped honey from photos of hive frames. We applied these methods to a large field study replicated across two continents, where we treated hives with the neonicotinoid imidacloprid and the acaricide thymol, alone and in combination. We found that the effect of imidacloprid on bee colonies varied between environments and that the miticide thymol can have long-term negative effects on bee colonies. We further investigated how these pesticides affect bee colonies by studying the effects of thymol on the hygienic behaviour of honeybees, and the effect of imidacloprid on individual bee foraging performances. These results add to our understanding of bee colony growth and development and how honey bee colony performance is affected by pesticides, and will help improve honeybee health worldwide.
Table of ContentsChapter 1. General introduction -- Chapter 2. Using within-day hive weight changes to measure environmental effects on honey bee colonies -- Chapter 3. The development of honey bee colonies assessed using a new semi-automated brood counting method : CombCount -- Chapter 4. Long-term dynamics of honey bee colonies following exposure to chemical stress -- Chapter 5. The miticide thymol in combination with the neonicotinoid imidacloprid reduces visual learning performance in European honey bees -- Chapter 6. Effects of thymol on European honey bee hygienic behaviour -- Chapter 7. Traces of a neonicotinoid induce precocious foraging and reduces foraging performance in honey bees -- Chapter 8. General discussion.
NotesIncludes bibliographical references Empirical thesis.
Awarding InstitutionMacquarie University
Degree TypeThesis PhD
DegreePhD, Macquarie University, Faculty of Science and Engineering, Department of Biological Sciences
Department, Centre or SchoolDepartment of Biological Sciences
Year of Award2019
Principal SupervisorAndrew B. Barron
Additional Supervisor 1William Dean Meikle
RightsCopyright Théotime Colin 2019. Copyright disclaimer: http://mq.edu.au/library/copyright
Extent1 online resource (255 pages) colour illustrations
Former Identifiersmq:72099 http://hdl.handle.net/1959.14/1281355