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Generation and analysis of zebrafish expressing KillerRed to induce oxidative stress in zebrafish models of amyotrophic lateral sclerosis

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thesis
posted on 28.03.2022, 23:53 by Anuj Dhoj Raut
Amyotrophic Lateral Sclerosis (ALS) is an incurable neurodegenerative disorder characterized by the muscle wasting, paralysis, hyperreflexia, and spasticity. Zebrafish has emerged as a powerful model to study various diseases, including ALS. Moreover, ALS transgenic zebrafish have been developed to study ALS. Oxidative stress has been hypothesis as a cause of neuron death in neurodegenerative diseases including ALS. However, the lack of experimental tools to target oxidative stress in specific cells has prevented direct evaluation of the hypothesis. We attempted to use a KillerRed, a genetically-encoded protein that generates reactive oxygen species (ROS) upon light activation, to induce oxidative stress in the zebrafish motor neurons and ubiquitously. We have shown its efficacy in live zebrafish and have applied KillerRed to stress motor neurons. Thus, we established a method to address the role of oxidative stress in an ALS model Zebrafish.

History

Table of Contents

Section 1.0. Introduction -- Section 2.0. Hypothesis and aim -- Section 3.0. Materials and methods -- Section 4.0. Results.

Notes

Theoretical thesis. Bibliography: pages 73-82

Awarding Institution

Macquarie University

Degree Type

Thesis MRes

Degree

MRes, Macquarie University, Faculty of Human Sciences, Australian School of Advanced Medicine

Department, Centre or School

Australian School of Advanced Medicine

Year of Award

2014

Principal Supervisor

Nicholas Cole

Rights

Copyright Anuj Dhoj Raut 2014. Copyright disclaimer: http://mq.edu.au/library/copyright

Language

English

Extent

1 online resource (83 pages) colour illustrations

Former Identifiers

mq:51586 http://hdl.handle.net/1959.14/1121727