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Improved larval diets for mass rearing of Queensland fruit fly (Bactrocera tryoni)

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posted on 28.03.2022, 11:36 by Tahereh Moadeli
Queensland fruit fly (Bactrocera tryoni, (Froggatt), aka Q-fly), is one of the most damaging horticultural pests in Australia. Sustainable methods are needed for Q-fly management. Sterile insect technique (SIT), in which millions of sterile flies are released to reduce pest reproduction, is effective. However, more efficient larval diet formulations are needed for production of high quality flies in a cost-effective mass rearing system. This thesis was conducted with the aim of developing high productivity larval diets for Q-fly mass rearing, and providing new insights into economical and nutritional aspects of larval diet formulation and Q-fly nutritional requirements. Two formulations of 'gel diet' were developed, and these new diets outperformed both traditional solid diets and more recently developed liquid diets, with outstanding benefits for rearing both at laboratory and factory scale. Subsequent investigations improved these formulations by identifying economical and effective yeasts (a source of amino acids) and plant oils (a source of lipids), as well as exploring the roles of specific nutritional components of yeasts and plant oils (fatty acid, vitamin E and amino acid contents). The initial and refined formulations were then investigated over multiple generations, providing further demonstration of the effectiveness of gel diets for mass rearing. In conclusion, the aims of the project were achieved and the gel diets that were developed have been implemented in the new SIT plus fruit fly mass-rearing facility in Port Augusta (South Australia), where they are already producing more than 20 million flies each week. The formulations of gel diet that have been developed for mass rearing of Q-fly could be modified and further developed for mass rearing of other fruit flies around the world, providing an efficient and economical solution for other SIT programs as well as providing a valuable tool for basic studies of nutrition.

History

Table of Contents

Chapter 1. General introduction -- Chapter 2. High productivity gel diets for mass rearing of Queensland fruit fly, Bactrocera tryoni -- Chapter 3. Comparison of gel larval diet with traditional lucerne chaff and carrot solid diets for rearing of Queensland fruit fly -- Chapter 4. Effects of wheat germ oil concentration in gel larval diets on production and quality of Queensland fruit fly, Bactrocera tryoni (Diptera: Tephritidae) -- Chapter 5. Effects of fatty acid and vitamin E in larval diets on development and performance of Queensland fruit fly -- Chapter 6. Canola oil as an economical lipid source in gel larval diet for Queensland fruitfly -- Chapter 7. Evaluation of yeasts in gel larval diet for Queensland fruit fly, Bactrocera tryoni -- Chapter 8. Multi-generational evaluation of gel larval diet formulations for Queensland fruit fly -- Chapter 9. General conclusions -- Appendices.

Notes

Includes bibliographical references Thesis by publication.

Awarding Institution

Macquarie University

Degree Type

Thesis PhD

Degree

PhD, Macquarie University, Faculty of Science and Engineering, Department of Biological Sciences

Department, Centre or School

Department of Biological Sciences

Year of Award

2019

Principal Supervisor

Phillip Taylor

Additional Supervisor 1

Fleur Ponton

Additional Supervisor 2

Peter Crisp

Rights

Copyright Tahereh Moadeli 2019. Copyright disclaimer: http://mq.edu.au/library/copyright

Language

English

Jurisdiction

Queensland

Extent

1 online resource (iv, 157 pages)) colour illustrations, 1 colour map

Former Identifiers

mq:72227 http://hdl.handle.net/1959.14/1282676