Proteomics of Pseudomonas aeruginosa cystic fibrosis isolates to understand host adaptation

Pseudomonas aeruginosa is an opportunistic bacterial pathogen commonly associated with cystic fibrosis (CF) lung infection. Numerous studies have investigated the mechanism of adaptation of P. aeruginosa to CF lungs, predominantly using the laboratory reference strain PAO1 as a model organism. However, considering the vast diversity of P. aeruginosa strains isolated from CF patients, additional information is needed to understand the role of specific proteins in this pathophysiology. This thesis describes proteomic-oriented investigations of novel CF isolated P. aeruginosa strains (PASS1-4) to better understand how this organism adapts to the harsh microenvironment, typical of the CF lung.