Pseudomonas aeruginosa is an opportunistic bacterial pathogen commonly associated with cystic fibrosis (CF) lung infection. Numerous studies have investigated the mechanism of adaptation of P. aeruginosa to CF lungs, predominantly using the laboratory reference strain PAO1 as a model organism. However, considering the vast diversity of P. aeruginosa strains isolated from CF patients, additional information is needed to understand the role of specific proteins in this pathophysiology. This thesis describes proteomic-oriented investigations of novel CF isolated P. aeruginosa strains (PASS1-4) to better understand how this organism adapts to the harsh microenvironment, typical of the CF lung.
Table of ContentsChapter-1: Introduction -- Chapter-2: Proteomics of host and pathogens in cystic fibrosis -- Chapter-3: Genetically and phenotypically distinct Pseudomonas aeruginosa cystic fibrosis isolates share a core proteomic signature -- Chapter-4: Quantitative proteomics by SWATH-MS reflects alterations in the phenotypic profiles of Pseudomonas aeruginosa strains isolated from cystic fibrosis patients -- Chapter-5: Pseudomonas aeruginosa cell membrane protein expression from phenotypically diverse cystic fibrosis isolates demonstrate host specific adaptations -- Chapter-6: TMT and SWATH proteomic analyses reveal adaptation dynamics of Pseudomonas aeruginosa to the hypoxic environment -- Chapter-7: General discussions -- Chapter-8: References -- Chapter-9: Appendix.
NotesBibliography: pages 173-196
Awarding InstitutionMacquarie University
Degree TypeThesis PhD
DegreePhD, Macquarie University, Faculty of Science and Engineering, Department of Chemistry and Biomolecular Sciences
Department, Centre or SchoolDepartment of Chemistry and Biomolecular Sciences
Year of Award2017
Principal SupervisorMark Molloy
Additional Supervisor 1Ian T. Paulsen
Additional Supervisor 2Helena Nevalainen
RightsCopyright Karthik Shantharam Kamath 2017.
Copyright disclaimer: http://mq.edu.au/library/copyright
Extent1 online resource (xv, 228 pages) illustrations