Pseudomonas aeruginosa is an opportunistic bacterial pathogen commonly associated with cystic fibrosis (CF) lung infection. Numerous studies have investigated the mechanism of adaptation of P. aeruginosa to CF lungs, predominantly using the laboratory reference strain PAO1 as a model organism. However, considering the vast diversity of P. aeruginosa strains isolated from CF patients, additional information is needed to understand the role of specific proteins in this pathophysiology. This thesis describes proteomic-oriented investigations of novel CF isolated P. aeruginosa strains (PASS1-4) to better understand how this organism adapts to the harsh microenvironment, typical of the CF lung.
Table of Contents
Chapter-1: Introduction -- Chapter-2: Proteomics of host and pathogens in cystic fibrosis -- Chapter-3: Genetically and phenotypically distinct Pseudomonas aeruginosa cystic fibrosis isolates share a core proteomic signature -- Chapter-4: Quantitative proteomics by SWATH-MS reflects alterations in the phenotypic profiles of Pseudomonas aeruginosa strains isolated from cystic fibrosis patients -- Chapter-5: Pseudomonas aeruginosa cell membrane protein expression from phenotypically diverse cystic fibrosis isolates demonstrate host specific adaptations -- Chapter-6: TMT and SWATH proteomic analyses reveal adaptation dynamics of Pseudomonas aeruginosa to the hypoxic environment -- Chapter-7: General discussions -- Chapter-8: References -- Chapter-9: Appendix.Notes
Bibliography: pages 173-196
Theoretical thesis.Awarding Institution
Macquarie UniversityDegree Type
Thesis PhDDegree
PhD, Macquarie University, Faculty of Science and Engineering, Department of Chemistry and Biomolecular SciencesDepartment, Centre or School
Department of Chemistry and Biomolecular SciencesYear of Award
2017Principal Supervisor
Mark MolloyAdditional Supervisor 1
Ian T. PaulsenAdditional Supervisor 2
Helena NevalainenRights
Copyright Karthik Shantharam Kamath 2017.
Copyright disclaimer: http://mq.edu.au/library/copyrightLanguage
EnglishExtent
1 online resource (xv, 228 pages) illustrationsFormer Identifiers
mq:70540
http://hdl.handle.net/1959.14/1265276