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Release properties of melatonin loaded mesoporous silica materials

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thesis
posted on 29.03.2022, 03:38 authored by Irene Moroni
Melatonin is a hormone produced by the pineal gland and involved in the regulation of circadian rhythms (e.g. sleep/wake cycle). Exogenous melatonin is used in the treatment of circadian disorders (e.g. delayed sleep phase syndrome, jet-lag or shift-work). In currently available commercial formulations melatonin has low bioavailability. Tailored formulations are needed to treat diverse and specific circadian disorders. Mesoporous silica materials can be efficient drug carriers as they are non-toxic biodegradable materials possessing high surface area, large pore volume and pore dimensions in the mesoscale range. This study focused on the preparation of mesoporous silica formulations for controlling the release of melatonin. Mesoporous materials (MCM-41) were synthesised and characterized through different physicochemical techniques. They were loaded with melatonin and their release properties were investigated in simulated gastro and intestinal fluids under sink conditions. Results suggested that MCM-41 alone did not delay the release of melatonin significantly. Thus, the particles were coated with polyacrylic acid (PAA) to further slow the rate of release. Preliminary biological assays revealed that both uncoated and PAA-coated MCM-41 with melatonin did not significantly increase apoptosis nor ROS production compared to the control (no treatment) when added for 24 h to mouse microglial cells (BV2).

History

Table of Contents

Chapter 1. Introduction -- Chapter 2. Materials and methods -- Chapter 3. Results -- Chapter 4. Conclusions -- Chapter 5. Future work -- References -- Supporting information.

Notes

Bibliography: pages i-iv Empirical thesis.

Awarding Institution

Macquarie University

Degree Type

Thesis MRes

Degree

MRes, Macquarie University, Faculty of Science and Engineering, Department of Molecular Sciences

Department, Centre or School

Department of Molecular Sciences

Year of Award

2017

Principal Supervisor

Alfonso Garcia-Bennett

Additional Supervisor 1

Maria Comas Soberats

Rights

Copyright Irene Moroni 2017. Copyright disclaimer: http://mq.edu.au/library/copyright

Language

English

Extent

1 online resource (v, 47, i-iv, a-c pages) illustrations

Former Identifiers

mq:71234 http://hdl.handle.net/1959.14/1272206