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Rose Bengal-conjugated gold nanoparticles ;: quantification of singlet oxygen generation in photodynamic therapy

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posted on 28.03.2022, 23:55 by Manoj Badrinath Kale
Photodynamic therapy (PDT) is an advanced novel methodology for the treatment of cancers using photosensitizers (PSs) which upon irradiating light generate cytotoxic singlet oxygen (¹O₂) and kills cancer cells. The utilization of photosensitizers has some restrictions, like low coefficient of extinction, low ¹O₂ generation efficiency and cannot be used for treating deep-seated tumours. Nanoparticles (NPs) have various properties that can be applied in PDT to overcome the limitations of PSs. The main aim of this work is, therefore, to enhance the ¹O₂ generation efficiency of the photosensitizer Rose Bengal (RB) by conjugating it with the Gold nanoparticles (AuNPs). The quantitative measurement of ¹O₂ generation is carried out on photosensitizer RB, AuNPs, and Au-NPs conjugated RB (AuRB). The fluorescence probe Singlet Oxygen Sensor Green (SOSG) was used to detect the ¹O₂. In a systemic study, the dependence of fluorescence on AuRB conjugation with different PS concentrations and the light-irradiation time for ¹O₂ generation are performed. Finally, the singlet oxygen quantum yield (SOQY) measurements are carried from the calculation of the rate of reaction and discussed. The results of the project state the improvement in ¹O₂ generation efficiency of PS on conjugation with the NPs. The successful NP-PS conjugation and high SOQY give the motivation for further studies on the development of the new photosensitizer drugs to treat the cancer cells.

History

Table of Contents

1. Introduction -- 2. Photodynamic therapy background -- 3. Instrumentation and analysis methods for nanomaterials characterization -- 4. Experimental procedure, results and discussion -- 5. Conclusions and future works -- List of acronyms/abbreviations -- References.

Notes

Bibliography: pages 44-50 Empirical thesis.

Awarding Institution

Macquarie University

Degree Type

Thesis MRes

Degree

MRes, Macquarie University, Faculty of Science and Engineering, Department of Physics and Astronomy

Department, Centre or School

Department of Physics and Astronomy

Year of Award

2016

Principal Supervisor

Ewa M. Goldys

Rights

Copyright Manoj Badrinath Kale 2016. Copyright disclaimer: http://mq.edu.au/library/copyright

Language

English

Extent

1 online resource (xii, 50 pages) diagrams, graphs

Former Identifiers

mq:69677 http://hdl.handle.net/1959.14/1256648