posted on 2022-03-28, 19:38authored byNicole Ewa Walczyk
"Several parameters associated with climate change, in particular elevated atmospheric CO2 concentrations, influence peanut plant growth. Commercial peanut plants grown in different regions of Australia are exposed to different environmental conditions. After extensive evaluation of methods to analyse the allergenicity of peanuts on a protein level, projected CO2 concentrations and different growth regions in Australia were tested for their impact on the abundance of peanut allergens. While the impact of elevated CO2 could not be determined conclusively, different environmental growth conditions did not appear to have an impact on the allergenicity of peanuts. Peanuts from different growing regions in Australia had a vet similar allergen content. The extraction method strongly influenced allergen extraction efficiency and their subsequent detection, which shows that the standardisation of peanut protein extraction is essential to quantify peanut allergenicity and understand its molecular and physiological basis." -- Abstract.
History
Table of Contents
1. Introduction -- 2. Methods and general optimisations -- 3A. Effect of different buffers and their pH values on extraction yield of peanut proteins and allergens Ara h 1 and 2 -- 3B. Evaluation of common peanut protein extraction parameters and determination of allergen content -- 4. Impact of elevated atmospheric CO₂ during plant growth on the allergen content of peanuts -- 5. Protein and Ara h1 and 2 content of peanuts grown in different regions in Australia -- 6. General discussion, conclusions and future directions.
Notes
Includes bibliographical references
A dissertation submitted in fulfilment of the requirements for the degree of Doctor of Philosophy, Department of Chemistry and Biomolecular Sciences, Faculty of Science, Macquarie University".
"June 2012
Awarding Institution
Macquarie University
Degree Type
Thesis PhD
Degree
PhD, Macquarie University, Faculty of Science, Department of Chemistry and Biomolecular Sciences