01whole.pdf (30.62 MB)
Download file

The impact of elevated carbon dioxide concentration and other environmental conditions on the allergenicity of peanuts

Download (30.62 MB)
thesis
posted on 28.03.2022, 19:38 authored by Nicole Ewa Walczyk
"Several parameters associated with climate change, in particular elevated atmospheric CO2 concentrations, influence peanut plant growth. Commercial peanut plants grown in different regions of Australia are exposed to different environmental conditions. After extensive evaluation of methods to analyse the allergenicity of peanuts on a protein level, projected CO2 concentrations and different growth regions in Australia were tested for their impact on the abundance of peanut allergens. While the impact of elevated CO2 could not be determined conclusively, different environmental growth conditions did not appear to have an impact on the allergenicity of peanuts. Peanuts from different growing regions in Australia had a vet similar allergen content. The extraction method strongly influenced allergen extraction efficiency and their subsequent detection, which shows that the standardisation of peanut protein extraction is essential to quantify peanut allergenicity and understand its molecular and physiological basis." -- Abstract.

History

Table of Contents

1. Introduction -- 2. Methods and general optimisations -- 3A. Effect of different buffers and their pH values on extraction yield of peanut proteins and allergens Ara h 1 and 2 -- 3B. Evaluation of common peanut protein extraction parameters and determination of allergen content -- 4. Impact of elevated atmospheric CO₂ during plant growth on the allergen content of peanuts -- 5. Protein and Ara h1 and 2 content of peanuts grown in different regions in Australia -- 6. General discussion, conclusions and future directions.

Notes

Includes bibliographical references A dissertation submitted in fulfilment of the requirements for the degree of Doctor of Philosophy, Department of Chemistry and Biomolecular Sciences, Faculty of Science, Macquarie University". "June 2012

Awarding Institution

Macquarie University

Degree Type

Thesis PhD

Degree

PhD, Macquarie University, Faculty of Science, Department of Chemistry and Biomolecular Sciences

Department, Centre or School

Department of Chemistry and Biomolecular Sciences

Year of Award

2013

Principal Supervisor

Thomas Roberts

Rights

Copyright disclaimer: http://www.copyright.mq.edu.au Copyright Nicole Ewa Walczyk 2013.

Language

English

Extent

1 online resource (xiii, 177 pages) illustrations, map +

Former Identifiers

mq:28202 http://hdl.handle.net/1959.14/267666 2066104