Macquarie University
01whole.pdf (13.37 MB)

Understanding biofilm development on diverse interfaces and controlled environments using novel microfluidic devices

Download (13.37 MB)
posted on 2023-10-23, 04:55 authored by Ye Zhang

Biofilms are ubiquitous structured communities of bacterial cells encased in a selfproduced polymeric matrix that protects the microorganisms from hostile physical and chemical environments that grow and respond to stimuli from internal biological processes and external environmental conditions. Biofilms can attach to different surface types and could be beneficial or detrimental depending on the microbial species and their growth. Among all the environmental factors, the interfaces in which biofilm grows play a critical role that directly affects how they thrive and interact with the environment. While there are many devices available to culture biofilm for in vitro studies, such as the microtiter plate, the Calgary Biofilm Device, the Center for Disease Control reactor, and the rotating disk reactor, most of these platforms are somewhat limited to the modelling of solid-liquid interface (SLI) biofilms, where the biofilms are attached to solid-rigid platforms submerged in liquid. Indeed, the environment in which biofilms thrive is diverse, and they could grow on air-liquid interface (ALI), liquidliquid interface (LLI), solid-air interface (SAI) etc. For example, biofilms found along the respiratory tracts and on the roots or leaves of plants are ALI biofilms, whereas biofilms in the urinary tract and on porous membranes that separate different media, such as those commonly found in wastewater treatment, can be best represented as LLI biofilm. Despite the plethora of scientific studies on biofilm, a detailed description of how biofilm develops on different interfaces in a well-controlled environment remains scarce. Being able to control the environment is important to meaningfully compare the different properties and growth patterns exhibited by the biofilm models developed on different interfaces. A device with an inclusive platform that enables the change in interfaces while allowing for the precise manipulation of growth conditions and environmental factors would also be useful and important to determine the efficacy of biofilm control methods accurately.

The studies in this thesis have been broadly categorised into four sections, specifically: (1) the design and development of a microfluidic device that enables the use of different interfaces for biofilm culture, (2) the application of the microfluidic device for the dynamic control of environmental conditions to understand biofilm growth on different interfaces, (3) the design and development of a device that enables the real-time in situ electrochemical monitoring of biofilm growth, and (4) demonstration on how the aforementioned devices can be used to access the efficacy of novel drug formulations for treating biofilm.


Macquarie University Research Excellence Scholarship


Table of Contents

Chapter 1 Introduction -- Chapter 2 Dual chamber microreactor for biofilm culture on diverse interface types -- Chapter 3 Dynamic dual-chamber microfluidic platform for biofilm study -- Chapter 4 Real-time and in situ electrochemical monitoring of biofilms growth -- Chapter 5 Application of the novel dual-chamber microfluidic device for assessing novel antimicrobial drugs -- Chapter 6 Conclusions, limitations and future work -- Bibliography

Awarding Institution

Macquarie University

Degree Type

Thesis PhD


Doctor of Philosophy

Department, Centre or School

School of Engineering

Year of Award


Principal Supervisor

Shaokoon Cheng

Additional Supervisor 1

Hui Xin Ong

Additional Supervisor 2

Ming Li


Copyright: The Author Copyright disclaimer:




208 pages

Former Identifiers

AMIS ID: 253429