Macquarie University
01whole.pdf (2.61 MB)

Withstanding heat waves: proteomic analysis of adaptive thermotolerance in Eucalyptus grandis seedlings

Download (2.61 MB)
posted on 2022-03-28, 15:25 authored by Timothy M. Maher
Heat wave events have been implicated in an increasing number of large scale die-off events in eucalypt-dominated forest throughout Australia. Being sedentary, plants in part rely on the expression of a specific set of proteins for heat acclimation and survival. However, the specific patterns of protein expression that underlie variability in important ecological traits like thermotolerance are rarely investigated in native plant species. This study tested the capacity of six provenances of Eucalyptus grandis (Flooded Gum) arrayed along a strong temperature gradient to respond to experimental manipulations of heat shock. Recently sequenced, E. grandis is a dominant species in forests along the east coast of Australia and a key species in forestry plantings. A comparative proteomic approach was used to characterise types and abundances of proteins involved in the heat shock response between seedlings grown from different provenances. We focused on seedlings as they are most vulnerable to intense heat conditions at the soil surface and to have limited access to soil moisture due to shallow root systems. Synthesis: Seedlings from southern provenances that experience more frequent heat stress, demonstrated higher levels of acquired thermotolerance and may have greater adaptive capacity to cope with increased heat waves under climate change.


Table of Contents

Introduction -- Materials and methods -- Experimental design -- Results -- Appendices.


Bibliography: pages 51-57 Empirical thesis. "This thesis is written in the form of a journal article from the Journal of Ecology adhering to most of the author guidelines (Appendix II) apart from instances where the guidelines go against the Macquarie University thesis formatting requirements" -- page 2

Awarding Institution

Macquarie University

Degree Type

Thesis MRes


MRes, Macquarie University, Faculty of Science and Engineering, Department of Biological Sciences

Department, Centre or School

Department of Biological Sciences

Year of Award


Principal Supervisor

Ian Alexander Wright

Additional Supervisor 1

Rachael V. Gallagher

Additional Supervisor 2

Mehdi Mirzaei


Copyright Timothy M. Maher 2016. Copyright disclaimer:




1 online resource (67 pages) illustrations (some colour), maps (some colour)

Former Identifiers