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Isolation of cancer cells on nanomaterial functionalized microfluidic devices

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thesis
posted on 28.03.2022, 01:14 authored by Bhuwan Ghimire
Primary cancerous tumors release cells that either stay locally or metastasize (spread) to different tissues through blood, referred to as circulating tumor cells (CTCs). CTCs are extremely rare in patients with metastatic cancer in initial stages (one cell per 109 hematologic cells in blood approx.) Isolation of circulating tumor cells (CTCs) is widely pursued as these cells provide valuable information about the metastasis and invasiveness of cancer growth through protein expression and is a critical element for therapeutic monitoring. Conventional batch processing methods do not provide precise safe handling and spatial-temporal manipulation for CTCs. Enrichment of CTCs with microfluidic methods provides a non-surgical, non-invasive and cost-effective alternative for CTC isolation through analysis of body fluids like blood or urine in case of bladder cancer. This thesis presents the fabrication, optimization and capture analysis results of a microfluidic device developed for capturing DU145, a prostate cancer cell line. Potential of the nanomaterial graphene oxide has been investigated in capture of these cells via immuno-affinity binding with the MIL-38 antibody inside the device. Binding affinity of antibodies towards the functionalized surfaces has been explored. The capture rates for DU145 and C3 cell lines on graphene oxide functionalized and non-functionalized surfaces are presented in this thesis.

History

Table of Contents

1. Introduction -- 2. Experimental methods : materials, instrumentation and analytical tools -- 3. Results and discussions -- 4. Conclusions and future works -- References -- Appendix.

Notes

Bibliography: pages 45-50 Empirical thesis.

Awarding Institution

Macquarie University

Degree Type

Thesis MRes

Degree

MRes, Macquarie University, Faculty of Science and Engineering, Department of Physics and Astronomy

Department, Centre or School

Department of Physics and Astronomy

Year of Award

2019

Principal Supervisor

Lianmei Jiang

Additional Supervisor 1

Jim Piper

Rights

Copyright Bhuwan Ghimire 2018. Copyright disclaimer: http://mq.edu.au/library/copyright

Language

English

Extent

1 online resource (viii, 51 pages) illustrations (some colour)

Former Identifiers

mq:70983 http://hdl.handle.net/1959.14/1269662