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Novel fluorescent natural products for biotechnology

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thesis
posted on 28.03.2022, 09:53 by Tahnim Sultana
Fluorescent natural products are becoming of interest in the fields of medicine, biotechnology and environmental science. However, while nature is a souce of a vast number of bioactive compounds very few fluorescent probes have been discovered from natural products. This research is addressing the discovery of new fluorescent pigments and to expand its application into a new domain. A fungus, Epicoccum nigrum, was chosen for this project. E. nigrum is the sole member of the genus Epicoccum (family Pleosporaceae) and gets its name from its dark colour. The colour is produced by red, blue and purple pigments, one of them ( epicocconone) has been commercialised as a total protein stain for proteomics (Deep Purple; GE Healthcare), a fluorescent stain for live cell imaging (LavaCell; Fluorotechnics) and a protein quantification solution (Fluoroprofile; Sigma-Aldrich). This project aims to isolate new pigments from this fungus through the optimisation of growth conditions on agar plates and moving to medium scale fermentation, extraction, purification and structure elucidation of other pigments.

History

Table of Contents

1 Introduction -- 2 Experimental -- 3 Results and discussion -- 4 Conclusion -- 5 References -- 6 Supplementary Materials.

Notes

Bibliography: pages 35-59 Theoretical thesis.

Awarding Institution

Macquarie University

Degree Type

Thesis MRes

Degree

MRes, Macquarie University, Faculty of Science and Engineering, Department of Chemistry and Biomolecular Sciences

Department, Centre or School

Department of Chemistry and Biomolecular Sciences

Year of Award

2017

Principal Supervisor

Peter Karuso

Rights

Copyright Tahnim Sultana 2017 Copyright disclaimer: http://mq.edu.au/library/copyright

Language

English

Extent

1 online resource ( vii, 52 pages pages)

Former Identifiers

mq:71464 http://hdl.handle.net/1959.14/1274609

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